Journal:

Article Title: SK3 is an important component of K + channels mediating the afterhyperpolarization in cultured rat SCG neurones

doi: 10.1111/j.1469-7793.2001.00323.x

Figure Lengend Snippet: A, concentration-inhibition curves for block of rSK3 current in transfected CHO cells by UCL 1684 (⋄), UCL 1530 (▵), dequalinium (○) and gallamine (□). Each point is the mean of 3 observations and the vertical bars indicate the s.e.m. The lines were drawn by fitting the Hill equation to the data with nH= 1. The IC50 values were: UCL 1684 5.8 ± 0.3 nm, UCL 1530 195 ± 34 nm, dequalinium 920 ± 140 nm and gallamine 97.5 ± 0.7 μm. B, correlation between log IC50 for block of rSK3 current (ordinate) and of the AHP (abscissa). The regression line shows a close correlation (r = 0.99± 0.21) between the potency of the compounds in the two test systems with a slope close to unity (1.06 ± 0.05). IC50 values for inhibition of the AHP in SCG neurones for UCL 1530, dequalinium and gallamine are taken from Dunn et al. (1996); all other values are shown above and in Table 1. With the exception of UCL 1530, the error in each point was less than the size of the symbol.

Article Snippet: SCG neurones SCG neurones were cultured from 17-day-old Sprague-Dawley rats using the procedure described by Dunn (1994) .

Techniques: Concentration Assay, Inhibition, Blocking Assay, Transfection

Journal:

Article Title: SK3 is an important component of K + channels mediating the afterhyperpolarization in cultured rat SCG neurones

doi: 10.1111/j.1469-7793.2001.00323.x

Figure Lengend Snippet: The potency of blocking agents tested on the SCG AHP and on SK channels

Article Snippet: SCG neurones SCG neurones were cultured from 17-day-old Sprague-Dawley rats using the procedure described by Dunn (1994) .

Techniques: Blocking Assay